The Hacienda of Nuestra Señora de los Dolores de El Carro, formerly known as Hacienda de Espíndola de Ciénaga Grande, was part of the Ciénaga de Mata estate and was located within the jurisdictions of Aguascalientes, Charcas, and Sierra de Pinos.

The town of Villa Ocampo, located beside the Bocas River, was an old Jesuit mission that was part of the Spanish Crown's evangelization efforts in the northern viceroyalty. Initially, its role was to provide a place for travelers along the Royal Inland Road to rest and seek shelter as they journeyed toward the northern settlements of Nueva Vizcaya.

The establishment of the mining district Noria de San Pantaleón in 1556 was the result of the work being carried out in the San Martín mines, located in the present-day state of Zacatecas. These mines were important producers of lead, which was required in the region's mines as it was necessary for smelting metals.

One of the first mining towns established north of Zacatecas was the town of San José de Avino, discovered by Captain Francisco de Ibarra in 1554. However, its exploitation began several years later, around 1630. Despite this, it was abandoned for long periods and was re-exploited around 1730 by the Basque José de Echaez. It was during this time that the name "Real de San José del Avinito" was formally adopted.

Jesuit missionaries were the founders of the mining towns of San Antonio de Cuencamé and Mapimí. This religious order was also responsible for spreading the veneration of the Señor de Mapimí in these areas, which became one of the most important religious traditions in Nueva Vizcaya since the 18th century.

The town of Nombre de Dios is considered the oldest settlement in Durango and is a significant point along the Royal Inland Road due to its contribution to the colonization process of northern Mexico.

During the early stages of the northward expansion into what would become New Spain, specifically between the 1520s and 1540s, the town of Aculco and its surroundings were a significant transit area for colonizers and missionaries. For this reason, this part of the Royal Inland Road was referred to as the "Gate of Tierra Adentro," through which the regular movement of merchants, officials, ranchers, and livestock took place.

The stretch between the Puente de La Colmena and the Ex Hacienda de la Cañada connected the towns of Tepeji del Río and Jilotepec and still retains both the original route layout and the cobblestone pavement made of round stones. This 3.5 km long route is flanked by small stone walls and large pirules (a type of tree).

The stretch of the Royal Inland Road between Nazas and San Pedro del Gallo is located in an area that was part of the presidio line, on the borders of the Bolsón de Mapimí. It spans approximately fifty kilometers, connecting the Hacienda de San Antonio, the former Jesuit mission of Cinco Señores (now Nazas), and the presidio of San Pedro del Gallo. These points were part of the route between Cuencamé and Parral and represented a day's journey on foot for travelers.

The need to transport silver extracted from the mines of Zacatecas to the central part of the country, particularly to Mexico City, for smelting and minting influenced the emergence of the stretch of the Royal Inland Road between the city of Zacatecas and the town of Ojocaliente.

An important agricultural center and historical city where in 1811, in the San Francisco Monastery, the heads of the fathers of Mexican Independence were put on display, to the horror of the local population: Miguel Hidalgo, Ignacio Allende, Juan Aldama and Mariano Jiménez.

The Villa Protectora de San Miguel el Grande and Sanctuary of Jesús Nazareno de Atotonilco is located in the north-central region of Guanajuato, which before the arrival of the Spanish was occupied by Chichimec Indians extending from Chamacuero to Guanajuato and San Felipe. The first contacts between the natives and the Spanish occurred in the 1520s, but colonization would not take place until 1542.