The Camino Real de Tierra Adentro includes five sites already inscribed on the World Heritage List and 55 other sites distributed along 1,400 of the 2,600 km of this long route that starts in northern Mexico and reaches Texas and New Mexico in the United States. Used between the 16th and 19th centuries, this road was used to transport silver extracted from the mines of Zacatecas, Guanajuato and San Luis Potosí, as well as mercury imported from Europe. Although its origin and use are linked to mining, the Camino Real de Tierra Adentro also fostered the establishment of social, cultural and religious links between Hispanic and Amerindian cultures.

This was an important religious center as shown by the circular foundations of its temples, a pyramid with a sunken courtyard, ballcourts, altars and terraces, plus mural and cave paintings. Believed to have been the most populous and extensive site in the Guadiana Valley.

Constructed in 1899 as a hospital, this national heritage building is now used by various institutions and includes the exhibition galleries of the National Institute of Anthropology and History (INAH) for the state of Durango, with a large collection of objects on the pre-Hispanic Chachihuite culture and a space for temporary exhibitions.

This most important and influential site of the Valley of Guadiana is related to the Chichimeca and coastal peoples of Mesoamerica. This museum reveals its rich, 1400-year history with displays on the Chalchihuite’s environment, religion and daily life.

This was the capital of Nueva Vizcaya in New Spain and a prosperous city during the 18th century because it was located on the Camino Real de Tierra Adentro (the Royal Inland Road). It has been the scene of battles such as the occupation of the city during the Mexican Revolution, and is currently noted for its cultural and artistic festivals.

The interior of the Cueva de las Mulas houses rock art associated with the presence of mules and other animals brought by the Spaniards conquistadors to the region during the 14th and 17th centuries. It is located in the Molino Canyon, in the Guatimapé Valley, within the municipality of Nuevo Ideal, in the state of Durango.

The Ojuela mine was founded in 1598 and is currently located in the municipality of Mapimí. It flourished during the 18th century and was part of a system of mines and real estates that was crucial in the route of the Royal Inland Road.

The stretch of the Royal Inland Road between Nazas and San Pedro del Gallo is located in an area that was part of the presidio line, on the borders of the Bolsón de Mapimí. It spans approximately fifty kilometers, connecting the Hacienda de San Antonio, the former Jesuit mission of Cinco Señores (now Nazas), and the presidio of San Pedro del Gallo. These points were part of the route between Cuencamé and Parral and represented a day's journey on foot for travelers.

The town of Villa Ocampo, located beside the Bocas River, was an old Jesuit mission that was part of the Spanish Crown's evangelization efforts in the northern viceroyalty. Initially, its role was to provide a place for travelers along the Royal Inland Road to rest and seek shelter as they journeyed toward the northern settlements of Nueva Vizcaya.

La Hacienda de la Limpia Concepción de El Canutillo, located in the current municipality of Ocampo, Durango, dates back to the second half of the 17th century. Originally built as an agricultural estate, its primary function was to supply the mining center of Parral (Chihuahua). However, it eventually became one of the wealthiest haciendas in the region due to its proximity to the Río Florido, which facilitated irrigation and thereby increased productivity.