The museum displays objects from the Tlaxcalan culture found in excavations of areas close to the pre-Hispanic temple. The culture is characterized by its codex-style polychrome ceramics, which portray an important cult to Tezcatlipoca with representations of human sacrifice rituals.

The building was fitted out in 1991 to store the archeological collection amassed from the excavation seasons to that date. It has two exhibition galleries and a room for research. It has around 750 pieces including stone and clay sculptures, obsidian arrow tips, bone needles, spindle whorls (malacates) and an important collection of ceramics. The display also includes burials with offerings.

The building has wide stone walls and a tiled roof. The facade and back wall are decorated with details from pre-Hispanic mural paintings and polychrome ceramics that have been found at the site.

The Ocotelulco site museum has a garden with plants used in pre-Hispanic times such as various species of agave, nopal and avocado. There are also trees which came to this land with the arrival of the Spanish, such as peaches, lemons and oranges.