In the viceregal period, convent churches reserved an upper level opposite the main altar for the choir. This was where the religious community gathered to chant psalms, hymns, and prayers at designated hours, following the rules of their order. Some of these choir lofts, such as the one in the Colegio de Guadalupe, were furnished with carved wooden choir stalls adorned with Marian symbols. Between each stall stand full-body sculptures of various saints. At the center, a wooden lectern (facistol) held large choir books containing the community’s sung prayers.

Though the artists responsible for Guadalupe’s magnificent ensemble remain unknown, the quality of the carving and polychromy can still be admired in its original location. Among the depicted saints are apostles holding the instruments of their martyrdom, interspersed with Marian symbols from the Litany of Loreto. At the center stands a life-size sculpture of Saint Francis of Assisi. Unlike the other painted and gilded sculptures, this one was designed to be dressed and includes a mechanism allowing it to be positioned seated or standing, depending on the needs of various ceremonies.

The walls of this space are adorned with large-format paintings that reflect Franciscan devotions, including Saint Joseph and a Franciscan allegory depicting the battle against heresy, located on the main wall.